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– Nelson Mandela

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Lab Module 8 biochemical tests

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Q 1.1. Phenol-Red Fermentation Assays a. What are two common by-products of sugar fermentation? How is phenol-red broth designed to measure both? b. What does a pink-red color after incubation indicate about the bacterium of interest? Explain your conclusion.

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a. The two common by-products of sugar fermentation are hydrogen and carbon dioxide . During fermentation , any gas that is produced by the microbes get trapped in the inverted Durham tube and bubbles will appears. The color change of the medium changes from red to yellow that is if red is there was no acid production. b. The pink color shows that the results came out as negative which means that the bacteria did not ferment the carbohydrates.